Fig. 4.

Disruption of mitochondrial thioredoxin system shortens lifespan of long-lived mitochondrial mutants. Deletion of trx-2 (A,B) or trxr-2 (C,D) significantly decreased the lifespan of nuo-6 and isp-1 worms but had little or no effect on the lifespan of wild-type worms. Disruption of trx-1 markedly decreased lifespan in nuo-6, isp-1 and wild-type worms (E,F). Deletion of trxr-1 significantly decreased nuo-6 lifespan but did not decrease the lifespan of wild-type worms (G). isp-1 trxr-1 double mutants could not be generated due to close proximity on the same chromosome. Statistical significance was assessed using a log-rank test. p-values indicate significance of difference between red and purple lines. A minimum of three biological replicates were performed. Raw lifespan data can be found in Table S1. A bar graph of this data can be found in Fig. S5. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)