Fig. 5.

Disruption of mitochondrial thioredoxin system increases sensitivity to oxidative stress in long-lived mitochondrial mutants. Deletion of trx-2 significantly decreases the survival of nuo-6 (A) and isp-1 (B) worms under chronic oxidative stress resulting from exposure to 4 mM paraquat but has little effect in wild-type worms. Disruption of trxr-2 also decreased paraquat survival in nuo-6 (C) and isp-1 (D) worms while increasing survival in wild-type animals. Deletion of trx-1 decreased the paraquat survival of nuo-6 (E) and isp-1 (F) worms but had no effect in wild-type worms. Disruption of trxr-1 decreased the paraquat survival of nuo-6 worms, but increased survival in wild-type animals (G). Statistical significance was assessed using a log-rank test. p-values indicate significance of difference between red and purple lines. Three biological replicates were performed. Error bars indicate SEM. Raw data can be found in Table S1. A bar graph of this data can be found in Fig. S5. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)