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Isolation and verification of GSCs and GSC-derived EVs. (A) Immunofluorescence assay identified the GSCs surface markers, SOX2 (red) and Nestin (Green). (B) The purification of GSC-derived EVs was verified by the exosome markers CD63. (C) The morphology of GSC-derived EVs was visualized by transmission electron microscopy and (D) NTA analysis was taken to analyze the characterization of EVs.
