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. 2022 May 23;12:8666. doi: 10.1038/s41598-022-12718-z

Figure 2.

Figure 2

Comparison of S. aureus growth and biofilm formation in standard and acidic media. (a) Pathogen growth curves in standard LB and acidic media (pH = 4.3). Data are expressed as mean ± SD. All the experiments (n = 4) were repeated 3 times on different days. OD = optical density. HA = hydrochloric acid. LA = lactic acid. (b) Biofilm viability assay in standard and acidic media after 24, 48, and 72 h incubation. Data are expressed as mean ± SD of 3 independent experiments (n = 4). RFU = relative fluorescence units. (c) Representative 2D and 3D images of crystal violet (CV) stained S. aureus biofilm at 72 h grown in LB and acidic media. 2D pictures magnification: 460X. Scale bar: 100 μm.