Figure 1.

Expression of AAV viruses in the lumbar spinal cord of wild-type Wistar adult rats. (A) Western blots of the images confirming expression of two viruses, AAV2.1-mDlx-GCaMP6f-Fishell-2 (N = 3 rats) and AAV9-hSynapsin-soCoChR-GFP (N = 3 rats) with beta-actin as the reference protein. (B) Confirmation of expression was performed by visual inspection of the fluorescent reporter, where after immunohistochemistry was performed and cell colocalization and counting was performed in three dorsoventral regions of the lumbar cord (D, 560 $\times$ 720  $\mathrm{\mu }{\text{m}}^2$), central (C, 590 $\times$  1070 $\mathrm{\mu }{\text{m}}^2$) and ventral (V, 460 $\times$ 770 $\mathrm{\mu }{\text{m}}^2$). Red color represents GAD65, green GFP (mDlx), cyan GAD67 and blue DAPI. Full length western blots are shown in the supplementary Fig.  1A,B.