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. 2022 May 23;12:8627. doi: 10.1038/s41598-022-12535-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The AAV2-mDlx enhancer primarily expressed in GAD65-containing neurons in the lumbar spinal cord. (A) Sample tissue section and illustration of the spinal cord showing a distinct layer of GAD67-containing synaptic terminals and neurons (cyan) whereas GAD65-containing neurons are more dispersed (red). The AAV2-mDlx-driven viral expression is shown in green (GFP). (B) A highlighted section (panel A) indicates co-localization of GFP and GAD65, but not GAD67. There are also instances of GAD65+ cells where GFP was not expressed (dim arrow). (C,D) Fraction of co-localization of GFP+ cells and cells containing NeuN (C, N = 2 rats, n = 3 sections/rat) and GAD65 (D, N = 3 animals, n = 3 sections) in the three dorso-ventral regions. (E) The dense layer of GAD67-containing neurons in the dorsal horn (substantia gelatinosa) has little or no overlap with GFP. (F) Cell count (shown as %) of the co-expression in the three regions between GAD67 and GFP positive cells (N = 3 animals, n = 3 sections/animal).