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. 2022 May 23;12:8627. doi: 10.1038/s41598-022-12535-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AAV2-mDlx has expression in GABAergic neurons, specifically the GAD65+ neurons. (A) Top row: original images of mDlx reporter (GFP, green) and GAD65 (red) and GAD67 (cyan) and a colocalization pixel map of GFP/GAD65 (right). Middle row: thresholded versions of top row, with the overlap in heat map (right) (scale bar 20 $μ$ m). Bottom row: overlap of GFP/GAD65/GAD67, GFP/GAD65 and GFP/GAD67, respectively. (B,C) Varying the threshold and calculating the co-localization as correlation (C). Note N = 4 animals, n = 3 sections/animal.