Figure 4.

Infection of spinal neurons with AAV9-CamKIIa primarily in the central and ventral regions. (A) Overview of infection (red, mScarlet designated here as RFP) in the half spinal section combined with the immunostaining of VGluT1 (green) and 2 (cyan) and zoomed in images of a highlighted region in half section and the immunostaining of VGluT1 and 2 (green and cyan), indicates some overlap with VGluT1, whereas the VGluT2 primarily stains the synaptic terminals. (B) Counting neurons (NeuN) that co-expressed with the cells expressing RFP (N = 6 animals, n = 3 sections/animal). (C) Box plot of the co-expressed cell count with VGluT1 positive neurons (N = 3 animals, n = 3 sections/animal). Scale bar 200 $\mathrm{\mu }$m in image A (left) and 20 $\mathrm{\mu }$m in the insets.