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. 2022 May 23;12:8627. doi: 10.1038/s41598-022-12535-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AAV9-CamKIIa has little or no colocalization with VGluT2 and VGluT1 staining. (A) Top row: the infected cells with the CamKIIa promoter (AAV9) express mScarlet (RFP) and its overlap with VGluT2 (cyan), and second row: thresholded views of the top row and the heat map of RFP and VGluT2. (B) Top right grey box: fluorescent correlation plots of RFP and VGluT2 show very little co-expression. (C) First row: original images showing mScarlet (RFP), VGluT1 (green) and their overlap, and bottom row: the threshold views of the images and and the heat map for co-localization. (D) Bottom right grey box: the correlation for various levels of threshold appear dark. (N = 3 animals, n = 3–4 sections/animal). Scale bar 20 $μ$ m.