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. 2022 May 23;12:8627. doi: 10.1038/s41598-022-12535-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AAV9-CamKIIa-infected cells has poor overlap with GAD65 and GAD67 biomarkers. (A) Top row: mScarlet expression (red, RFP) and the immunostaining of GAD65 (cyan) has only little co-expression, and second row: threshold representations of the original images and a heat map co-expression plot of RFP/GAD65. (B) Top right grey box: plots show poor correlation in pixel-overlap between RFP and GAD65. (C) First row: original images of RFP, GAD67 (green) and their overlap, and bottom row: threshold representations of the original images and the heat map of RFP/GAD67. (D) Bottom right grey box: the mosaic of correlation coefficients for various thresholds, indicate poor overlap between RFP and GAD67. (N = 3 rats, n = 3-4 sections/rat). Scale bar 20 $μ$ m.