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. 2022 May 23;12:8627. doi: 10.1038/s41598-022-12535-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AAV9-hSyn1 targets a large fraction of spinal neurons. (A) A spinal section with infection using hSyn1-promoter co-stained with NeuN marker for neurons. (B) Highlighted region in (A) shows the extensive overlap between the infected cells and the NeuN marker and box plots at the bottom right show 40–60% co-expression of NeuN and GFP in the dorsal region whereas 90% overlap in the central and ventral regions. Scale bar = 200 $μ$ m in image (A) and 20 $μ$ m in the zoomed-in images (B). N = 4 animals, n = 3 sections/animal.