Unique stem cell line identifier	SCVIi028-A-1
Alternative name(s) of stem cell line	SCVi-15S1-PE2
Institution	Stanford University School of Medicine
Contact information of the reported cell line distributor	Ioannis Karakikes, ioannis1@stanford.edu
Type of cell line	iPSC
Origin	Human
Additional origin info	Age: 21
	Sex: Male
	Ethnicity: Not Hispanic or Latino
Cell Source	PBMCs
Method of reprogramming	SendaI Virus (CytoTune™-iPS 2.0 Sendai Reprogramming Kit)
Clonality	Clonal (Isolated using the Isocell supplied by iotaSciences to ensure mono-clonality)
Evidence of the reprogramming transgene loss (including genomic copy if applicable)	PCR, Western Blot, qPCR for sendai virus - negative
Cell culture system used	Matrigel-coated feeder-free culture, StemMACS iPS-Brew XF media
Type of Genetic Modification	Transgene generation
Associated disease	N/A
Gene/locus	AAVS1 (OMIM 102699)/ 19q13
Method of modification/site-specific nuclease used	CRISPR/Cas9
Site-specific nuclease (SSN) delivery method	RNP
All genetic material introduced into the cells	sgRNA for AAVS1 locus, pAAVS1-PE2-P2A-BFP donor plasmid
Analysis of the nuclease-targeted allele status	PCR for WT allele and Sanger sequencing and confirmation of integration by junction PCR
Method of the off-target nuclease activity surveillance	In silico prediction and Targeted PCR with Sanger sequencing
Name of transgene	PE2-P2A-BFP [Cas9(H840A)MMLV RT-P2A-BFP]
Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific)	Positive (neomycin, and Dox-inducible puromycin)
Inducible/constitutive system details	TET-On
Date archived/stock date	07/2021
Cell line repository/bank	https://hpscreg.eu/cell-line/SCVIi028-A-1
Ethical/GMO work approvals	Patient was enrolled in the study by informed consent approved by the Stanford Institutional Review Board (IRB # 29904)
Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable)	pAAVS1-NDi-CRISPRi (Gen1) was a gift from Bruce Conklin (Addgene plasmid # 73497 ; http://n2t.net/addgene:73497 ; RRID:Addgene_73497) pU6-Sp-pegRNA-HEK3_CTT_ins was a gift from David Liu (Addgene plasmid # 132778 ; http://n2t.net/addgene:132778 ; RRID:Addgene_132778) pTRE3G-PE2-P2A-BFP was a gift from Jesse Engreitz and Glen Munson.