Table 4.

Experimental matrix and response values from randomized runs in the Box–Behnken design

Run	Code variable level			Real variable level (g/L)			Inhibition rate (%)
	B	C	E	Corn flour	Beef extract	MgSO4
1	− 1	1	0	9	18	13	79.8
2	0	− 1	− 1	11	14	12	80.9
3	− 1	0	− 1	9	16	12	74.6
4	1	0	− 1	13	16	12	86.7
5	0	0	0	11	16	13	97.4
6	1	− 1	0	13	14	13	86.7
7	0	− 1	1	11	14	14	83.8
8	− 1	− 1	0	9	14	13	82.7
9	1	1	0	13	18	13	79.3
10	0	0	0	11	16	13	88.3
11	0	1	− 1	11	18	12	83.7
12	− 1	0	1	9	16	14	78.2
13	1	0	1	13	16	14	74.8
14	0	0	0	11	16	13	87.9
15	0	1	1	11	18	14	71.5
16	0	0	0	11	16	13	88.7
17	0	0	0	11	16	13	87.2