Figure 4.

Maternal MHC class I molecules are dispensable for the development of uNK cells. (A) Mating strategy to assess the impact of MHC class I on uNK by comparing wildtype females with β2m^-/- mice that lack surface expression of MHC-I, both mated with B6 males. (B, C) Comparison of abundance of all uNK cells in terms of absolute number per implantation site (B), and relative frequency among CD45⁺ cells (C). uNK numbers were measured at gestational age Total uNK were gated as shown in Figure 3A . (D) Relative abundance of the 2 main subsets of uNK within NK1.1⁺ NKp46⁺ Eomes⁺ cells. (E, F) Phenotypic assessment of uNK for Ki-67 (E) which is associated with cell proliferation and KLRG1 which marks terminal maturation and correlates with education in cNK cells (F). Data representative of 3 experiments with n = 6-7 mice per group. Means ± SEM. P-values from unpaired two-tailed Student’s t-tests. See also Figure S1 .