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. 2022 May 10;13:808227. doi: 10.3389/fimmu.2022.808227

Figure 8.

Figure 8

Educated uNK cells do not harm fetuses lacking self MHC class I (A) Mating strategy to assess the effect of absent placental/fetal MHC class I expression in MHC sufficient hosts. (B) Enumeration of fetuses either sufficient or deficient for MHC class I surface expression carried by MHC sufficient females. (C) Weight distribution of fetuses either sufficient or deficient for MHC class I surface expression carried by MHC sufficient females. Data representative of 11 litters. (D) Experimental layout to assess ability of heterozygous β2m+/- mice to reject MHC class I-deficient haematopoietic cells (splenocytes). (E) Representative histograms showing in vivo rejection of MHC class I deficient splenocytes by MHC class I sufficient hosts. (F) Comparison of specific rejection of MHC-deficient conceptuses and splenocytes. Means ± SEM. Data representative of 84 offspring (A-C) and 5 mice per experiment (D-F). P-value from an unpaired two-tailed Student’s t-test. CFSE, Carboxyfluorescein succinimidyl ester.