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. 2022 May 10;13:890019. doi: 10.3389/fimmu.2022.890019

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Copyright © 2022 Li, Li, Wu, Xu, Teng, Yang, Sun, Zhao, Li, Liu, Yang, Gong and Cai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identifying myeloid cell subpopulations in liver tissue. (A) Clustering of 4883 myeloid cells and cell annotation. (B) tSNE plots colors by spatial distribution of cells in normal (n=3) and liver transplantation (n=4) human tissues. (C) tSNE plots colors by spatial distribution of cells in 7 samples. (D) Dotplots of each cluster of myeloid cells’ top 5 marker genes. (E) Gene expression of Kupffer cells’ canonical markers across myeloid cells. (F) Pseudotime analysis with identified 3 Kupffer groups. (G) Gene expression of dendritic cells’ canonical markers across myeloid cells. (H) Pseudotime analysis with 5 dendritic cell groups.