TABLE 1.
Lethal insertion targets
| Clonea | Gene(s) targetedb | Function category |
|---|---|---|
| G8 | RNA polymerase, beta | Transcription |
| B11 | Peptide release factor 2 | Translation |
| B4 | tRNA cluster operon | Translation |
| H10 | asp-tRNA synthetase | Translation |
| F7 | leu-tRNA synthetase | Translation |
| B6 | phe-tRNA synthetase | Translation |
| C1 | ser-tRNA synthetase | Translation |
| A2 | Genes encoding ribosome proteins (nusA-IF2 operon) | Translation |
| D6 | Genes of ribosome protein operon (S5, L30, SecY) | Translation |
| A6 | Genes of fatty acid synthesis operon (fabF, fabZ, accC, accD) | Membrane synthesis |
| H9 | Unknown | |
| D2 | Unknown |
Clone containing a lethal mutagenic plasmid. The insert sequences from these plasmids mapped (more than 95% identity) to the following positions on TIGR contigs: G8 (contig 4102, bp 10776 to 11079), B11 (contig 4113, bp 6008 to 6290), B4 (contig 4119, bp 2220 to 2504), H10 (contig 4230, bp 6722 to 6971), F7 (contig 4120, bp 8556 to 8820), B6 (contig 4142, bp 13006 to 13276), C1 (contig 4103, bp 363 to 622), A2 (contig 4151, bp 7602 to 7902), D6 (contig 4127, bp 12011 to 12300), A6 (contig 4200, bp 13076 to 13323), H9 (contig 4105, bp 670 to 932), and D2 (contig 4196, bp 2483 to 2707).
Sequences from G8 to A6 exhibited more than 50% identity to the sequences of the listed proteins or RNA from B. subtilis. Although we did not assign functions to the genes targeted by clones H9 and D2, the H9 sequence was less than 45% identical to the putative aspartate aminotransferase from B. subtilis and the D2 sequence was 35% identical to the putative carbonic anhydrase of Methanobacterium thermoformicicum.