Fig. 6. Truncated NV9 variants analyzed at 10 V.
The AUC is determined over the entire spectrum for the respective mass species, which is depicted along with error bars that represent the corresponding standard deviation. “dsA2” (yellow bars) corresponds to the empty complex, “dsA2/pep” (light blue bars) to dsA2 bound to one peptide, “dsA2/pep/pep” to dsA2 bound to two molecules of this certain peptide (dark blue bars) and “dsA2/erucamide” to dsA2 bound to the erucamide (coral) respectively. Octa- and nonapeptides have the highest binding efficiency in accordance with their biological function. Without the anchor residues Leu-2 and Val-9 in a truncated NV9 variant, the affinity decreases drastically. A terminal methionine appears to enhance the affinity of the peptide to dsA2. The corresponding Kd,low is shown for respective peptides.