Fig. 1.

Lineage tracing analysis of tdTomato-positive Sox9-lineage with Post regimen and immunohistochemistry of osteoblast markers at the 1-mm defect site in mouse calvaria bone defect model. Confocal microscopy images of coronal section in uninjured calvaria (left; sham), 3 days after 1-mm injured (middle; Day 3) and 10 days after 1-mm injured (right; Day 10) with tile scan and zoom images (n = 2 mice). The region between white arrow shows 1-mm defect. Sections were immunostained with antibodies for RUNX2 (middle) or SP7 (bottom). Scale bar, 1000 μm (white), 200 μm (yellow) and 100 μm (blue).