KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-PAX7 | DSHB | Cat# PAX7, RRID:AB_2299243 |
| Mouse monoclonal anti-MyoD | Santa Cruz Biotechnology | Cat# sc-377460, RRID:AB_2813894 |
| Mouse monoclonal anti-MyoG | DSHB | Cat# F5D, RRID:AB_2146602 |
| Mouse monoclonal anti-MyHC | DSHB | Cat# MF 20, RRID:AB_2147781 |
| Mouse monoclonal anti-eMyHC | DSHB | Cat# F1.652, RRID:AB_528358 |
| Rabbit polyclonal anti-Laminin | Sigma | Cat# L9393, RRID:AB_477163 |
| Rabbit polyclonal anti-Dystrophin | Abcam | Cat# ab15277, RRID:AB_301813 |
| Rabbit polyclonal anti-Ki67 | Abcam | Cat# ab15580, RRID:AB_443209 |
| Rabbit polyclonal anti-ATGL | Cell Signaling Technology | Cat# 2439, RRID:AB_2167953 |
| Mouse monoclonal anti-GAPDH | Santa Cruz Biotechnology | Cat# sc-32233, RRID:AB_627679 |
| PE Rat anti-mouse CD31 antibody | BD Biosciences | Cat# 553373, RRID:AB_394819 |
| PE anti-mouse CD45 antibody | eBioscience | Cat# 12-0451-82, RRID:AB_465668 |
| Pacific Blue anti-mouse Ly-6A/E (Sca-1) antibody | BioLegend | Cat# 122520, RRID:AB_2143237 |
| APC anti-mouse CD106 antibody | BioLegend | Cat# 105718, RRID:AB_1877141 |
| Alexa 568 goat anti-mouse IgG1 | Invitrogen | Cat# A-21124, RRID:AB_2535766 |
| Alexa 488 goat anti-mouse IgG1 | Invitrogen | Cat# A-21121, RRID:AB_2535764 |
| Alexa 647 goat anti-mouse IgG2b | Invitrogen | Cat# A-21242, RRID:AB_2535811 |
| Alexa 488 goat anti-rabbit IgG | Invitrogen | Cat# A-11034, RRID:AB_2576217 |
| Alexa 647 goat anti-rabbit IgG | Invitrogen | Cat# A-21244, RRID:AB_2535812 |
| HRP AffiniPure goat anti-mouse IgG | Jackson ImmunoResearch | Cat# 115-035-003, RRID:AB_10015289 |
| HRP AffiniPure goat anti-rabbit IgG | Jackson ImmunoResearch | Cat# 111-035-003, RRID:AB_2313567 |
| Chemicals, peptides, and recombinant proteins | ||
| Tamoxifen (TMX) | Calbiochem | 579000 |
| 4-Hydroxytamoxifen (4-OHT) | Sigma-Aldrich | H6278 |
| N-acetyl cysteine (NAC) | Sigma-Aldrich | A9165 |
| Evans blue | Sigma-Aldrich | E2129 |
| Cardiotoxin | Sigma-Aldrich | 217503 |
| Ketamine HCl | Akron | 59399-114-10 |
| Xylazine | Akron | 59399-110-20 |
| Collagenase, Type I | Worthington | LS004197 |
| Collagenase, Type II | Worthington | LS004179 |
| Dispase II | Sigma-Aldrich | 04942078001 |
| Zombie Violet Live/Dead dye | Biolegend | cat#423113 |
| Red blood cell lysis solution | Promega | Z3141 |
| Ham’s F-10 Nutrient Mix | Gibco | 11550043 |
| Dulbecco’s Modified Eagle Medium | Gibco | 11995065 |
| Fetal bovine serum | HyClone | SH30080.03 |
| Donor Horse Serum | Corning | MT35030CV |
| Opti-MEM | Gibco | 31985070 |
| Penicillin-Streptomycin | Sigma-Aldrich | P4333 |
| Seahorse XF base medium | Agilent Technologies | 103334-100 |
| Phosphate-buffered saline | Gibco | 21600-069 |
| Nile red | Invitrogen | N1142 |
| LipidSpot610 | Biotium | T0069 |
| CellROX Deep Red Reagent | Invitrogen | C10422 |
| pHrodo Red AM Intracellular pH Indicator | Invitrogen | P35372 |
| HCS LipidTOX™ Deep Red Neutral Lipid Stain | Invitrogen | H34477 |
| BODIPY 493/503 | Invitrogen | D3922 |
| BODIPY 558/568 C12 | Invitrogen | D3835 |
| M.O.M. (Mouse on Mouse) Blocking Reagent | Vector lab | MKB-2213 |
| Hoechst33342 | Invitrogen | H3570 |
| DAPI | Invitrogen | D1306 |
| Paraformaldehyde | Sigma-Aldrich | P6148 |
| Glycine | Sigma-Aldrich | 50046 |
| Sucrose | Fisher Scientific | S5-500 |
| Glucose | Sigma-Aldrich | G7021 |
| O.C.T. Compound | Fisher Scientific | 23-730-571 |
| Fibroblast growth factor, basic | Promega | 9PIG507 |
| BD Matrigel Matrix | BD Biosciences | 356235 |
| Collagen from rat tail | Sigma-Aldrich | C7661 |
| 5-Ethynyl-2′-deoxyuridine (EdU) | Cayman Chemical | 20518 |
| Tetramethylrhodamine (TAMRA) azide | Invitrogen | T10182 |
| A922500 | Sigma-Aldrich | A1737 |
| PF-06424439 | Sigma-Aldrich | PZ0233 |
| Atglistatin | Cayman Chemical | 15284 |
| Lipofectamine 2000 Transfection Reagent | Invitrogen | 11668030 |
| Glutardialdehyde | Sigma-Aldrich | 8.20603 |
| Sodium cacodylate | Sigma-Aldrich | 97068 |
| Osmium tetroxide | Sigma-Aldrich | 201030 |
| Uranyl acetate | Fisher Scientific | 18-607-644 |
| Ethanol | Decon Labs, Inc | 2701G |
| Acetonitrile | Fisher Scientific | A998-1 |
| EMbed-812 kit | Fisher Scientific | 50-980-391 |
| Goat serum | MP Biomedicals | 08642921 |
| Bovine serum albumin | GeminiBio | 700-105P |
| TRIzol Reagent | Sigma-Aldrich | T9424 |
| Chloroform | VWR Chemicals | BDH1109 |
| Methanol | Fisher Scientific | A412-20 |
| NP-40 | Thermo Scientific | 85124 |
| Sodium deoxycholate | Sigma-Aldrich | D6750 |
| Sodium Dodecyl Sulfate (SDS) | Fisher Scientific | 02-004-080 |
| Ammonium acetate | Fisher Scientific | A637-500 |
| Perchloric acid | Sigma-Aldrich | 244252 |
| PMSF | Calbiochem | 7110-OP |
| L-Glutamine solution | Sigma-Aldrich | G7513 |
| Sodium pyruvate | Sigma-Aldrich | P5280 |
| Etomoxir | Sigma-Aldrich | E1905 |
| Critical commercial assays | ||
| M-MLV reverse transcriptase | Invitrogen | 28025021 |
| PicoPure RNA Isolation Kit | Applied Biosystems | KIT0204 |
| FastStart Essential DNA Green Master | Roche | 06402712001 |
| Pierce BCA Protein Assay Reagent | Thermo Scientific | 23225 |
| Western Blotting Chemiluminescence Luminol Reagent | Santa Cruz Biotechnology | sc-2048 |
| PicoProbe Lipolysis Fluorometric Assay Kit | BioVision Inc | K578-100 |
| PicoProbe Acetyl-CoA Fluorometric Assay kit | BioVision Inc | K317 |
| ATP Detection Assay Kit | Cayman Chemical | 700410 |
| Seahorse XF Cell Mito Stress Test Kit | Agilent Technologies | 103015-100 |
| Chromium Single Cell 3′ GEM Library & Gel Kit v3 | 10x Genomics | PN-1000075 |
| Chromium Single Cell B Chip Kit | 10x Genomics | PN-1000153 |
| Chromium i7 Multiplex Kit | 10x Genomics | PN-120262 |
| Deposited data | ||
| Bulk RNA-seq data of WT and Pnpla2 KO satellite cells | This paper | GEO: GSE150632 |
| Single cell RNA-seq data of satellite cells during regeneration | This paper | GEO: GSE150366 |
| Experimental models: Organisms/strains | ||
| Mouse: B6;129-Pax7tm2.1(cre/ERT2)Fan/J | The Jackson Laboratory | JAX stock: #012476 |
| Mouse: B6.Cg-Pax7tm1(cre/ERT2)Gaka/J | The Jackson Laboratory | JAX stock: #017763 |
| Mouse: B6.129S4-Myf5tm3(cre)Sor/J | The Jackson Laboratory | JAX stock: #007893 |
| Mouse: B6;129-Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat/J | The Jackson Laboratory | JAX stock: #021039 |
| Mouse: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | The Jackson Laboratory | JAX stock: #007909 |
| Mouse: B6N.129S-Pnpla2tm1Eek/J | The Jackson Laboratory | JAX stock: #024278 |
| Mouse: D2.B10-Dmdmdx/J | The Jackson Laboratory | JAX stock: #013141 |
| Mouse: Pax7nGFP | Sambasivan et al., 2013 | N/A |
| Mouse: NOD-Rag1null IL2rgnull | Purdue University Biological Evaluation Core Facility | N/A |
| Oligonucleotides | ||
| See Table S3 for all primers | This paper | N/A |
| Recombinant DNA | ||
| pKMyc-ATGL | Xie etal., 2014 | N/A |
| Software and algorithms | ||
| Seurat v3.1 | Stuart et al., 2019 | Satija lab: satijalab.org/seurat/ |
| Cell Ranger v3.1 | 10x Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/3.0/#cellrangertab |
| RStudio | RStudio Team, 2015 | RRID:SCR_000432 |
| TopHat2 | Kim et al., 2013 | RRID:SCR_013035 |
| HTSeq V0.6.1 | Anders et al., 2015 | RRID:SCR_005514 |
| DESeq2 | Love et al., 2014 | RRID:SCR_015687 |
| BD FACSDiva Software | BD Biosciences | RRID:SCR_001456 |
| FlowJo 10 | FLOWJO, LLC | RRID:SCR_008520 |
| Fiji-ImageJ | Schindelin et al., 2012 | RRID:SCR_002285 |
| MetaMorph Microscopy Automation and Image Analysis Software | Molecular Devices, LLC | RRID:SCR_002368 |
| Agilent Masshunter Quantitative Analysis software | Agilent Technologies | RRID:SCR_015040 |
| Seahorse Wave | Agilent Technologies | RRID:SCR_014526 |
| Adobe Photoshop | Adobe Inc. | RRID:SCR_014199 |
| NIS-Elements | Nikon Instruments | RRID:SCR_014329 |
| ZEN 2010 Microscopy Software | Carl Zeiss Microscopy GmbH | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
| Prism 6.0 | GraphPad Prism | RRID:SCR_002798 |