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. 2022 May 18;3(2):101407. doi: 10.1016/j.xpro.2022.101407

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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High-content imaging controls and quality metrics

(A) Brightfield and fluorescent images of experiment positive and negative controls. Hoechst is used to identify the nuclei of all cells, Caspase-3/7 activated reagent is used to identify apoptotic cells, and TO-PRO-3 is used to identify all dead cells. Scale bar: 200 μm.

(B) Viability values from positive and negative control well replicates are used to assess assay quality by Z′-factor (left) and coefficient of variance (right). Each point represents the Z′-factor or coefficient of variance for each experiment plate from the 351-compound screen described in the companion publication (Larsen et al., 2021).