Passaging reagents

Reagent	Final concentration	Amount
TrypLE Express solution	1×	500 μL/well
PBS	1×	Variable
Dispase II (10 mg/mL PBS)	1×	250 μL/250 μL growth media
DNase I (5 mg/mL PBS)	10 K.U./mL	10 μL/10 mL PBS
Matrigel	100%	50 μL/well
TO Culture Media	1×	500 μL/well
10 mM Rock Inhibitor Y-27632	10 μM	1 μL/1 mL growth media