TABLE 2.
General features of putative ORFs contained on the pSK220 subclone of Tuc2009 and comparison to sequences from databases
| ORF | Start position (codon) | Stop position (codon) | Size of protein (kDa) | Function or similarity | Pa | Match (%)b | Accession no. (reference) |
|---|---|---|---|---|---|---|---|
| 10 | 4935 (ATG) | 4660 (TAG) | 9.9 | Unknown | |||
| 11 | 5038 (ATG) | 5253 (TAG) | 8.4 | ORF71 from φ31 | 1e−35 | 98 (71) | U51128 (11) |
| 12 | 5255 (ATG) | 5353 (TGA) | 3.7 | Unknown | |||
| 13 | 5369 (ATG) | 5887 (TGA) | 20.1 | Unknown | |||
| 14 | 5896 (ATG) | 6519 (TAA) | 24.0 | ORF11 from TP901-1 | 1e−109 | 92 (207) | Y14232 (27) |
| Gene e12 from bil170 | 2e−60 | 58 (203) | AF009630 (direct submission) | ||||
| ORF35 from sk1 | 3e−62 | 61 (203) | AFO11378 (3) | ||||
| 15 | 6519 (ATG) | 6971 (TAA) | 16.6 | Single-stranded binding protein from: | |||
| B. subtilis | 1e−37 | 67 (110) | P37455 (34) | ||||
| φ PVL | 1e−35 | 51 (157) | AB009866 (20) | ||||
| H. influenzae | 6e−14 | 37 (105) | U04997 (18) | ||||
| E. coli | 7e−12 | 41 (117) | g70816 (38) | ||||
| 16 | 7096 (GTG) | 7878 (TAA) | 29.3 | ORF11 from r1t | 4e−10 | 30 (156) | U38906 (44) |
| 17 | 7878 (ATG) | 8603 (TGA) | 27.0 | Unknown | |||
| 18 | 8600 (ATG) | 9358 (TAA) | 29.4 | Methyltransferase from: | |||
| Bacillus stearothermophilus | 5e−15 | 28 (231) | X97069 (7) | ||||
| Streptococcus pneumoniae | 9e−10 | 25 (259) | P09358 (8) | ||||
| Escherichia coli | 4e−10 | 46 (65) | U48806 (31) | ||||
| 19 | 9348 (TTG) | 9737 (TAA) | 15.8 | ORF14 from r1t | 2e−70 | 97 (128) | U38906 (44) |
| 20 | 9741 (ATG) | 9980 (TAA) | 9.4 | ORF16 from r1t | 2e−37 | 91 (79) | U38906 (44) |
| 21 | 10092 (ATG) | 10271 (TAA) | 6.9 | ORF17 from r1t | 7e−28 | 100 (59) | U38906 (44) |
| 22 | 10261 (ATG) | 10830 (TAG) | 22.4 | ORF18 from r1t | 3e−85 | 83 (189) | U38906 (44) |
| 23 | 10841 (ATG) | 11236 (TAA) | 15.5 | ORF115 from φg1e | 2e−04 | 31 (124) | X98106 (25) |
| 24 | 11229 (ATG) | 11774 (TGA) | 21.0 | ORF1 from L. lactis | 3e−18 | 37 (193) | Y11901 (36) |
| ORF19 from r1t | 7e−06 | 34 (121) | U38906 (44) |
a
Derived from BLASTP score; P, probability that the observed similarity had occurred by chance. Low P values indicate a high probability of two proteins being structurally related.
b
The number of amino acids over which the percentage match was determined is shown in parentheses.