Figure 5.

iBET impairs IC-associated DC chemotaxis in vivo. (A) Representative images showing movement of CD11c-YFP labelled dermal DCs in mice footpads by two-photon microscopy. Following treatment with iBET or solvent control, CD11c-YFP reporter mice were injected with Ova in one hind footpad and Ova-IC in the contralateral footpad, with imaging under isoflurane anaesthesia 18 hours later. Green shows DCs, yellow shows representative migration tracks of respective DCs. (B) Quantification of chemotaxis of dermal DCs in mice footpads. (C) Representative images showing movement of CD11c-YFP labelled dermal DCs and (D) quantification of chemotaxis in footpads of FcγRIIb -/- mice. Blood vessels labelled with Qdot^® probe (red), shown where successful. For all, medians are shown for data representative of 2 independent experiments, points show individual tracked dermal DCs. Significance testing using Mann-Whitney U test.