Figure 6.

iBET impairs IC-stimulated DC migration to lymph nodes. (A) Representative flow cytometry plots of draining lymph nodes of mice from FITC paint model. FITC paint was applied topically to shaved skin of mice to label dermal DCs, stimulated and treated with IC and iBET with appropriate controls, and draining lymph nodes were harvested 48 hours later. (B) Flow cytometry quantification of dermal DCs from draining and non-draining lymph nodes from FITC paint model. Medians are shown for data representative of 4 independent experiments, points show individual mice. (C) Lymph node cell number in FITC paint model following iBET treatment. (D) Diagram of experimental set up for murine BMDC transfer model. BMDC were cultured from fluorescent-labelled mice and treated with iBET or DMSO followed by transfer to wild-type mice. Recipient mice were culled 48 hours alter and lymph nodes were harvested. (E) Representative flow cytometry plot of CFP and GFP staining of DCs in draining lymph nodes. (F) Flow cytometry quantification of DC composition in draining lymph nodes. Data shown is representative of 10 mice from 3 independent experiments. Significance testing using Mann-Whitney U test (B, C) and Wilcoxon matched-pairs sign rank test (E).