FIGURE 8.

Steady-state TRPV4 activity is greater with reduced Ca²⁺ buffering (0.25 mM BAPTA compared to 2 mM BAPTA). (A) In a single cilium, the current-voltage relation was measured with 0.25 mM BAPTA (black), in the same solution after addition of the TRPV4 agonist GSK1016790A (50 nM, red), and in a solution with 50 nM GSK1016790A but with BAPTA increased to 2 mM (orange). (B) Paired currents activated by 50 nM GSK1016790A in 10 cilia, each tested in both 0.25 mM BAPTA and 2 mM BAPTA. The left plot shows current at −90 mV and the right at +90 mV *p = 0.0016 at both potentials.