FIGURE 9.

Activation of PC2 secondarily to a Ca²⁺ influx through TRPV4 channels. (A) In a single cilium with just one active PC2 channel, the ciliary current-voltage relation (left) was measured in the absence of the TRPV4 agonist GSK1016790A (black), in its presence (50 nM, red), and again in its absence (orange). Steady-state PC2 activity at −5 mV was reversibly activated by GSK1016790A (right). For each of the three recordings, a dashed line is shown at the level of the leak current (3.6 pA). The same cilium was used for the left and right figures. (B) A repeat of the experiment shown in (A) but using a cilium with multiple PC2 channels. The dashed lines again show the leak current (4.7 pA). (C) Paired mean PC2 currents at −5 mV after subtraction of leak currents in 8 cilia, each tested in the absence and presence of GSK1016790A. In all parts of this figure, Ca²⁺ was buffered with 0.25 mM BAPTA. *p = 0.0078.