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. 2022 Apr 20;298(6):101970. doi: 10.1016/j.jbc.2022.101970

Figure 1.

Figure 1

Endogenous 2AA formation and attack.A, reaction mechanism of serine/threonine dehydratase (IlvA) using serine as a substrate. B, pyruvate–PLP adduct is formed by 2-aminoacrylate attacking an active site PLP. Shown in the mechanism by which pyruvate–PLP is formed. A fold type III active site is represented with arginine as the residue coordinating the pyridine nitrogen of PLP (ala alanine racemase). The mechanism of attack has been described (2). Treatment with base releases a pyruvate–PLP adduct that can be easily separated from PLP and monitored by HPLC. 2AA, 2-aminoacrylate; 2IP, 2-iminopropanoate; PLP, pyridoxal 5′-phosphate.