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Alr is damaged when 2AA is present in vivo. Wildtype Alr was purified from wildtype (blue) and a ridA mutant (red) of S. enterica as described in the text. Cofactors from 0.4 mg of enzyme were released by treatment with base and separated by HPLC. Cofactors are labeled based on retention time and coinjection experiments with authentic standards. 2AA, 2-aminoacrylate.
