Table 3.
Primers
| Primer | Sequence (5’ – 3′) |
|---|---|
| Δpim1 FWD | GTTTAGTTGTTTTTTCTTTTGGTTTTCGAGGTGCTTGAACGAAAAGATTTGCAAATAGAGCatcttgaccgcagtt |
| Δpim1 REV | ATGTTTAAATATTTACAGAATGTTTAAACAGGTATTTAATCCATTTAGATGAAAAGTTAGTgtgtcgtttctattatgaatttc |
| YFH1-GFP FWD | CATTGTCCGGGCCTAACAGA |
| YFH1-GFP REV | CATACATACACACACGGTAC |
| Δpim1 check FWD | CCTTAGGATTCGAGAACTATGCAGAGGTGT |
| Δpim1 check REV | CCTGGCAAAACGACGATCTTCTTAGGG |
| Δpim1 check (-) REV | TACGTCACGCACGGTCAAGG |
| YFH1 FWD RT-qPCR | AGCGGTCTCTCGCAAGTTTA |
| YFH1 REV RT-qPCR | GCTGGACAAAATCTGGCACG |
|
FXN FWD RT-qPCR |
GCCTCAACCAGATTTGGAATGTC |
|
FXN REV RT-qPCR |
AGTCCAGCGTTTCCTCTGCTAG |
|
NRF2 FWD RT-qPCR |
ACACGGTCCACAGCTCATC |
|
NRF2 REV RT-qPCR |
TGTCAATCAAATCCATGTCCTG |
|
TXR FWD RT-qPCR |
GTAGTTGACTTCTCAGCCACGTG |
|
TXR REV RT-qPCR |
CTGACAGTCATCCACATCTACTTC |
|
GSR FWD RT-qPCR |
TATGTGAGCCGCCTGAATGCCA |
|
GSR REV RT-qPCR |
CACTGACCTCTATTGTGGGCTTG |
|
SOD2 FWD RT-qPCR |
CTGGACAAACCTCAGCCCTAAC |
|
SOD2 REV RT-qPCR |
AACCTGAGCCTTGGACACCAAC |
| ACTB FWD RT-qPCR | CAGCCTTCCTTCCTGGGTATG |
| ACTB REV RT-qPCR | AGGGTGTAAAACGCAGCTCA |
Abbreviations: FWD, forward; REV, reverse.