Table 4.
Key Resources Table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell Line (Homo sapiens) | WT fibroblasts | ATCC | ||
| Cell Line (Homo sapiens) | FRDA fibroblasts | Coriell | GM03816 | |
| Cell Line (Ratticus norvegicus) | H9C2 cardiomyocytes | Franklin Lab | ||
| Chemical compound, drug | Cycloheximide | Sigma | CAS# 66-81-9 | 125 μM |
| Chemical compound, drug | BPS (bathophenanthroline sulfonate) | Sigma | CAS#52746-49-3 | 80 μM |
| Chemical compound, drug | Ferrous sulfate | Sigma | CAS#7782-63-0 | 5 μM |
| Other | Dynabead M-280 Sheep anti-rabbit IgG | ThermoFisher | CAT#11203D | |
| Chemical compound, drug | Dimethyl pimelimidate | ThermoFisher | CAT#21667 | 20 mM |
| Chemical compound, drug | Triethanolamine | Sigma | CAS# 102-71-6 | 0.2 M |
| Chemical compound, drug | MitoSOX | ThermoFisher | CAT#M36008 | 5 μM |
| Chemical compound, drug | CDDO-Me | Sigma | CAS#218600-53-4 | 2 μM |
| Chemical compound, drug | Screen compound library | Microsource Discovery Systems | ||
| Commercial assay or kit | Agilent Tech. Mini RNA kit | Prod #5185-6000 | ||
| Antibody | Rabbit anti-GFP | Abcam | Ab6556 | |
| Antibody | Mouse anti-GFP | Sigma | SKU#11814460001 | .2 ug/107 beads |
| Antibody | Rabbit anti-Nfs1 (yeast) | 1:1000 | ||
| Antibody | Rabbit anti-Nfs1 (mammalian) | ThermoFisher | Ref# PA5-76984 | 1:1000 |
| Antibody | Rabbit antiyeast Isu1 | 1:5000 | ||
| Antibody | Rabbit antimammalian Iscu | ThermoFisher | CAT# PA5-70181 | 1:5000 |
| Antibody | Mouse antiyeast Porin | ThermoFisher | CAT#459500 | 1:5000 |
| Antibody | Rabbit anti-Fxn | Abcam | Ab175402 | 1:1000 |
| Antibody | Rabbit anti-GAPDH | Abcam | Ab9485-100 | 1:1000 |
| Antibody | Rabbit antiaconitase 2 | ThermoFisher | CAT# PA5-19269 | 1:5000 |
| Antibody | Rabbit anti-VDAC | ThermoFisher | CAT#PA1-954A | 1:10,000 |
| Antibody | Secondary peroxidase conjugated antibodies | Jackson Immuno Research |
1:1000 | |
| Commercial assay or kit | SuperSignal West Femto | ThermoFisher | CAT#34095 | 1:5000 |
| Commercial assay or kit | Western lighting plus ECL | PerkinElmer | NEL104001EA | |
| Software, algorithm | NIH Fiji | Imagej.net/fiji | ||
| Software, algorithm | GraphPad Prism Version 9.0.1 | www.graphpad.com |