Figure 1.

Validation of BRCA1 knockout in BRCA-KO clones. (A) Deleted and inserted nucleotides in BRCA1-KO clones giving rise to premature termination codons in the BRCA1 open reading frame. Sequences of guide RNA are indicated in green letters and red indicate nucleotide deletions (*) or insertions (N). (B) BRCA1 mRNA relative level in parental non-targeting control TNBC cells (sgNT), and two BRCA1-KO clones (BRCA1ko1 and BRCA1ko2). (C) Western blotting of BRCA1. β-actin was used as a loading control. Unpaired t‐tests were used in (B). Bars show the mean and the SEM of three independent experiments. *p-value<0.05; **p-value<0.01; ***p-value<0.001 and ****p-value<0.0001.