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. 2022 Mar 23;2(4):100125. doi: 10.1016/j.xjidi.2022.100125

Figure 4.

Figure 4

The effect of collagenases. (a) Representative data of CD4 and CD8 expression in dermal CD3+ T cells recovered with two different concentrations of collagenase type I (n = 2). (b, c) Representative data of (b) CD4 and CD8 expression and (c) CD69 and CD103 expression in dermal CD3+ T cells recovered with collagenase type IV (n = 2). (d) Representative data of CD4 and CD8 expression (left panels) and CD69 and CD103 expression (right panels) in subcutaneous fat CD3+ T cells recovered with either migration method or collagenase type I digestion. (e) A summary of MFI of CD8 (left) and CD69 (right) in subcutaneous fat CD3+ cells (n = 3). (f) Subcutaneous fat was incubated with PBS or dispase II overnight at 4 °C and then digested with collagenase type I. Representative data of CD4 and CD8 expression (left panels) and CD69 and CD103 expression (right panels) in subcutaneous fat CD3+ T cells. (g) A summary of MFI of CD8 (left) and CD69 (right) in subcutaneous fat CD3+ cells (n = 3). (h) Representative data of CD4 and CD8 expression (left panels) and CD69 and CD103 expression (right panels) in subcutaneous fat CD3+ T cells recovered with PBS or dispase II incubation overnight at 4 °C. (i) A summary of MFI of CD8 (left) and CD69 (right) in subcutaneous fat CD3+ cells (n = 3). Comparisons between groups were evaluated using Student’s t-test (one tailed). ∗∗P < 0.01. MFI, mean fluorescent intensity.