Figure 5.
The effect of dispase II. (a) Time course of in vitro generation of TRM-like T cells from PBMCs. (b) Representative expression of indicated antigens in in vitro generated TRM-like T cells before dispase II incubation (n = 3). (c, e) Representative expression of indicated antigens in in vitro generated TRM-like T cells after dispase II incubation. (d, f) A summary of MFI of the indicated antigens in in vitro generated TRM-like T cells after dispase II incubation (n = 3). Comparisons between groups were evaluated using Student’s t-test (one tailed). ∗∗P < 0.01 and ∗∗∗P < 0.001. MFI, mean fluorescent intensity; rhTGFβ, recombinant human TGFβ; TRM, resident memory T cells.