Figure 6.

Comparison between emigrants and the remaining T cells in the explants. Representative data of FSC versus SSC gating for (a) epidermis and (g) dermis. Lymphocyte populations are indicated by red circles (left panels). A summary of the percentage of lymphocytes (right panels; n = 4). A summary of absolute CD3⁺ T-cell count per 1 cm² of explants in (b, c) epidermis and (h, i) dermis (n = 5). A summary of the percentage of emigrants in the 1 cm² of explants in (d) epidermis and (j) dermis (n = 5). Summaries of the percentage of CD4⁺ or CD8⁺ cells in CD3⁺ cells for (e) epidermis and (k) dermis) (n = 3). Summaries of the percentage of CD103⁺ cells in (f) epidermis and (l) dermis (n = 3). Comparisons between groups were evaluated using two-way ANOVA test. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. D2, day 2; FSC, forward scatter; SSC, side scatter.