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. 2022 May 17;15:100294. doi: 10.1016/j.mtbio.2022.100294

Fig. 2.

Fig. 2

The cytotoxicity and biological function of PCP and NPs/miR365 nanocomplex. A) Cytotoxicity of PCP on MC38 ​cells at different concentrations (all data was compared with PCP at 0 ​μg ​mL−1 concentration). B) Cytotoxicity of 50 ​μg ​mL−1 of PCP on MC38 ​cells at different time points (compared with 0 ​h treatment). C) Cells uptake of NPs/miRNA in 293 ​T and MC38 ​cells. D) Inhibitory effect of NPs/miR365 on MC38 ​cells proliferation after 24 ​h treatment. PCP mediated miRNA mimic control (NPs/NC) and commercial lipofectamine 2000 mediated miR365 delivery (Lipo/miR365) were used as controls. E) The effects of NPs/miR365 on the proliferation of MC38 ​cells after 24 ​h, 48 ​h and 72 ​h treatment respectively. F) Live/dead staining of MC38 ​cells after 24 ​h treatment of NPs/miR365. G) Cell scratch images at 0, 12, 24 and 48 ​h were recorded using a microscope. H) Statistical analysis of scratch distance. n. s. (no significance). ∗P ​< ​0.05, ∗∗P ​< ​0.01, ∗∗∗P ​< ​0.001 (n ​= ​3).