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Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
