Skip to main content
. Author manuscript; available in PMC: 2022 May 24.
Published in final edited form as: Nat Protoc. 2021 Jan 18;16(2):1219–1250. doi: 10.1038/s41596-020-00456-3

Fig. 3 |. An overview of the Trigenic Synthetic Genetic Array (τ-SGA) experimental pipeline.

Fig. 3 |

MATα single and double mutant query strains are generated. A diagnostic array of MATa single mutant array strains is constructed. Query strains and array strains are crossed using τ-SGA method to generate double and triple mutants, which are scored for colony size, which is a proxy for fitness. Separately, fitness of the original query strains and array strains is measured. All the resulting fitness values are incorporated into the final quantification of digenic and trigenic interactions.