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. Author manuscript; available in PMC: 2022 May 24.
Published in final edited form as: Nat Protoc. 2021 Jan 18;16(2):1219–1250. doi: 10.1038/s41596-020-00456-3

Fig. 5 |. An example of a trigenic interaction.

Fig. 5 |

Top, sample cropped images of final τ-SGA plates. The deletion of the array gene (sla1Δ) alone or in combination with either one of the query genes (mtc1Δ or mdy2Δ) does not result in any observable fitness defects. The triple mutant exhibits a severe growth defect, and as such a strong negative trigenic interaction. Each mutant is represented in quadruplicate on the array and is highlighted with a black box. Bottom, tetrad analysis confirmation for the negative trigenic interaction of mtc1Δ mdy2Δ sla1Δ.