Reagent	Amount	Final concentration
H2O	74.2 μL	-
10x buffer	10 μL	1x
10 mM dNTPs	2 μL	200 μM
50 μM forward primer,	4 μL	2 μM
50 μM reverse primer	4 μL	2 μM
p4339 DNA template	0.1 μg in 0.5 μL H2O	1 ng/μL
DMSO	5 μL	5% vol/vol
5U/μL Taq DNA Polymerase	0.3 μL	0.015 U/ μL
Total	100 μL	-