Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Optimizations to reduce dsRNA contaminants include the use of different RNA polymerases, changes in experimental conditions (e.g., Mg2+ concentration or the use of selected modified nucleotides), and the use of manipulated-sequence-containing templates (e.g., deletion of U-rich sequence) during IVT. In the purification steps, HPLC or cellulose chromatography can be applied.
