Figure 3.

Hsp70 maintains FUS in the state of liquid-like droplets and prevents it from amyloid aggregation

(A) Representative images of the liquid-like droplets of FUS-EGFP in the absence or presence of Hsp70 in a maturation process of 18 h. Scale bar, 5 μm.

(B) FRAP images (left) and curves (right) of FUS-EGFP droplets in (a) are shown. Data are shown as mean ± SD with n = 3. Student’s t test, ∗ p < 0.05; ∗∗ p < 0.01; ns, not significant. Scale bar, 5 μm.

(C) The ThT fluorescence assay of FUS-LC (25 μM) with the addition of different concentrations of Hsp70. The molar ratios of FUS-LC to Hsp70 are indicated. Data correspond to mean ± SD, n = 3.

(D) Negative-staining TEM images of ThT samples at 110 h in (c). Scale bar: 500 nm. See also Figures S8 and S9.