Table 2.

Thermodynamic analysis of interactions

Syringe/cell	Temperature (°C)	Kd (nM)	N	ΔH (kJ mol−1)	−TΔS (kJ mol−1)	ΔG (kJ mol−1)
AtTAF4–RST/DREB2A	25	740 ± 320	1.24 ± 0.04	−5.19 ± 0.03	−29.8	−35.0
AtTAF4–RST/DREB2A	30	1050 ± 140	1.18 ± 0.01	−10.5 ± 0.2	−24.2	−34.7
AtRCD1–RST/DREB2Aa	25	16 ± 1	0.90 ± 0.00	−63.3 ± 0.2	18.7	−44.6
HsTAF4–TAFH/DREB2A	25	110 ± 50	1.15 ± 0.02	−15.4 ± 0.4	−24.1	−39.5
HsTAF4–TAFH/DREB2A	30	420 ± 120	0.85 ± 0.02	−23.9 ± 0.7	−12.9	–36.9
AtTAF4–RST/ANAC013	25	NB	NB	NB	NB	NB
AtTAF4–RST/ANAC013	30	1080 ± 630	0.68 ± 0.04	−1.8 ± 0.1	−32.7	−34.5
AtRCD1–RST/ANAC013b	25	9 ± 4	0.80 ± 0.01	−45.0 ± 0.8	−0.6	−45.6

Abbreviation: NB, no detectable binding.

Syringe/cell indicates whether the αα-hub domain or the TF is the titrant in the syringe or the titrant in the cell. The standard errors for ΔH, K_d, and N were obtained from Origin when fitting the data to a model of one set of binding sites.

^aData from Ref. (28).

^bData from Ref. (27).