The contrasting behavior between the CM, IM, and NCM subsets in the expression of genes involved in adhesion, homing, extracellular matrix (ECM) interactions, and tissue extravasation
Heatmap of select genes is shown on the top left. A gradient of high to low gene expression based on Z-score calculated from the average of normalized counts for healthy subjects and dengue patients in each monocyte subset, is shown from red to blue. Panels of bar graphs showing normalized expression read in CM, IM, and NCM subsets from healthy children and dengue patients and are grouped as per their bias toward CM, IM, or NCM. Each dot represents the normalized counts for the individual subject (healthy, n = 3; dengue, n = 6). Bars represent average reads in a given subset for a given condition. Blue: healthy children; Yellow: dengue febrile children. Error bars represent the SEM. For each bar graph, significant and non-significant differences within subsets from dengue patients (dCM, dIM and dNCM) and for dengue versus healthy comparison (D vs H) in each subset (CM, IM and NCM) is highlighted in a box, where ∗ denotes adjusted P-value <0.05; ∗∗ denotes adjusted P-value <0.01; ∗∗∗ denotes adjusted P-value <0.001; and “ns” denotes non-significant (also mentioned in Table S3G). The adjusted p values were obtained from DeSeq2 analysis.