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. 2022 May 11;4(3):fcac120. doi: 10.1093/braincomms/fcac120

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© The Author(s) 2022. Published by Oxford University Press on behalf of the Guarantors of Brain.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of Abca7 in the mouse brain. (A) In situ hybridization of Abca7 mRNA with digoxigenin-labelled antisense and sense probes and counterstained with nuclear Fast Red. (B) mRNA expression (Arbitrary Unit) of members of the Abca gene subfamily in the mouse brain (20 mg)— Abca1: 1.45, Abca2: 5.96, Abca3: 1.89, Abca4: 0.1, Abca5: 0.76, Abca6: 0.07, Abca7: 10.69, Abca8a: 0.22, Abca8b: 1.13, Abca9: 0.1, Abca12: 0.007, Abca13: 0.01. (C) Multiplex PCR genotyping of Abca7 knock mouse with neomycin replacing Abca7 exons 20 and 21. (D) Western blotting of Abca7 protein in the mouse brain. (E) Histology of the hippocampus of wild type and Abca7 knock mouse stained with 4′,6-diamidino-2-phenylindole.