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. 2022 May 20;3(2):101417. doi: 10.1016/j.xpro.2022.101417

Indexing PCR Master Mix

Reagent 96-well plate
384-well plate
1 well (μL) 1 plate (μL) 4 plates (μL) 1 well (μL) 1 plate (μL) 4 plates (μL)
KAPA HiFi DNA Polymerase 0.64 70.4 281.6 0.08 40 160
5× KAPA HiFi Fidelity Buffer 6.4 704 2816 0.8 400 1600
10 mM KAPA dNTP Mix 0.96 105.6 422.4 0.12 60 240
Nuclease-free water 1.6 176 704 0.2 100 400