Indexing PCR Program

Steps	Temperature	Time	Cycles
Initial Extension	72°C	3 min	1
Initial Denaturation	95°C	30 s	1
Denaturation	95°C	10 s	10 cycles
Annealing	55°C	30 s
Extension	72°C	1 min
Final extension	72°C	5 min	1
Hold	4°C	∞