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Lysis Buffer Master Mix
| Reagent | 96-Well plate |
384-Well plate |
||||
|---|---|---|---|---|---|---|
| 1 well (μL) | 1 plate (μL) | 10 plates (μL) | 1 well (μL) | 1 plate (μL) | 10 plates (μL) | |
| Nuclease-free water | 2.68 | 294.8 | 2948 | 0.335 | 134 | 1340 |
| RNase Inhibitor | 0.1 | 11 | 110 | 0.0125 | 5 | 50 |
| ERCC working solution | 0.1 | 11 | 110 | 0.0125 | 5 | 50 |
| 10% Triton | 0.02 | 2.2 | 22 | 0.0025 | 1 | 10 |
| 10 mM dNTP | 1 | 110 | 1100 | 0.125 | 50 | 500 |
| 100 μM oligo-dT30VN primer | 0.1 | 11 | 110 | 0.0125 | 5 | 50 |
