Reverse Transcription Master Mix
| Reagent | 96-Well plate |
384-Well plate |
||||
|---|---|---|---|---|---|---|
| 1 well (μL) | 1 plate (μL) | 4 plates (μL) | 1 well (μL) | 1 plate (μL) | 4 plates (μL) | |
| Nuclease-free water | 0.14 | 15.4 | 61.6 | 0.0175 | 8.75 | 35 |
| 5× first strand buffer (from Takara kit) | 2 | 220 | 880 | 0.25 | 125 | 500 |
| RNase inhibitor | 0.25 | 27.5 | 110 | 0.031 | 15.5 | 62 |
| 100 mM DTT (Invitrogen) | 0.5 | 55 | 220 | 0.063 | 31.5 | 126 |
| 1 M MgCl2 | 0.06 | 6.6 | 26.4 | 0.0075 | 3.75 | 15 |
| 5 M Betaine | 2 | 220 | 880 | 0.25 | 125 | 500 |
| 100 μM TSO | 0.1 | 11 | 44 | 0.0125 | 6.25 | 25 |
| SmartScribe II Reverse Transcriptase | 0.95 | 104.5 | 418 | 0.12 | 60 | 240 |