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. 2022 May 20;3(2):101417. doi: 10.1016/j.xpro.2022.101417

cDNA Amplification Master Mix

Reagent 96-Well plate
384-Well plate
1 well (μL) 1 plate (μL) 4 plates (μL) 1 well (μL) 1 plate (μL) 4 plates (μL)
Nuclease-free water 2.14 235.4 941.6 0.27 135 540
10 μM IS PCR primer 0.25 27.5 110 0.031 15.6 62.4
2× Kapa HiFi HotStart ReadyMix 12.5 1375 5500 1.56 780 3120
Lambda Exonuclease 0.11 12.1 48.4 0.014 7 28