Reagent | 96-Well plate |
384-Well plate |
||||
---|---|---|---|---|---|---|
1 well (μL) | 1 plate (μL) | 4 plates (μL) | 1 well (μL) | 1 plate (μL) | 4 plates (μL) | |
Nuclease-free water | 2.14 | 235.4 | 941.6 | 0.27 | 135 | 540 |
10 μM IS PCR primer | 0.25 | 27.5 | 110 | 0.031 | 15.6 | 62.4 |
2× Kapa HiFi HotStart ReadyMix | 12.5 | 1375 | 5500 | 1.56 | 780 | 3120 |
Lambda Exonuclease | 0.11 | 12.1 | 48.4 | 0.014 | 7 | 28 |